FIG. 4. .

Placental nitrotyrosine formation was not affected by genotype or treatment. A) Representative images are shown of nitrotyrosine staining in placental sections from C57BL/6J, Nos3^−/−, and Tempol-treated Nos3^−/− mice. Original magnification ×100. B) There were no difference between nitrotyrosine formation in placentas from C57BL/6J and that in Nos3^−/− mice. Furthermore, treatment of Nos3^−/− mice with Tempol had no affect on placental nitrotyrosine formation. Data show means ±SEM from n = 6–7 mice; one-way ANOVA; DAPI (blue) staining.