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Treatment with Tempol reduced vascular density in placentas from Nos3−/− mice. Vascular density, in ranges of 0–80 μm (A), 0–200 μm (B), and 0–500 μm (C), did not differ between placentas from C57BL/6J and those from Nos3−/− mice. Treatment of Nos3−/− mice with Tempol resulted in a significant decrease in vascular density in the 0–200 μm range (B). Line denotes median, n = 4–6 mice; Kruskal-Wallis test followed by Dunn multiple comparison test; *P < 0.05.
