Figure 6.

Combining NFV with BZ inhibits NSCLC and MM tumor growth in vivo. (a) H157 cells (upper) and RPMI8226 cells (lower) were grown as xenografts in athymic NCr-nu/nu. Bars, S.D. *P<0.05 for vehicle treatment, **P<0.005 for vehicle treatment. (b) Representative photos of RPMI8226 xenograft tumors of two different mice after 11 days of either treatment. Circles, RPMI8226 tumors. (c) ER stress, cytosolic proteotoxicity, and apoptosis markers in vivo. To examine the effect of the combination on biomarkers, H157 xenograft tumors after the 5 days of treatment as described in Materials and Methods were excised and prepared for the lysate. Levels of the indicated markers were assessed by immunoblotting. Densitometry was performed using NIH Image software, and levels of each marker were normalized to GAPDH for each sample; Columns, mean from all five mice examined. Bars, S.D. *P<0.05. (d) Inhibition of cell proliferation in H157 and RPMI8226 tumors. Levels of Ki67 were assessed by immunohistochemistry. The Ki67 staining index was achieved by assigning as described in Materials and Methods. Columns, mean from all five mice examined in H157 xenografts and from all three mice examined in RPMI8226 xenografts. Bars, S.D. *P<0.05