Figure 2.
Representative gels from 2D DIGE experiment, which included seven sera samples from patients without dementia (ND) and seven with dementia (HAD). Samples labeled with fluorescent dyes were resolved in pH gradient from 3 to 10 (first dimension IEF in 24 cm immobiline gels) and in gradient polyacrylamide gels 10–20% (second dimension SDS PAGE). After electrophoresis gels scanned at resolution of 100 μm and wavelength of 520 and 620 nm, optimal for Cy3 and Cy5 dyes respectively. A=ND; B=HAD. White circles and numbers shown in gel A correspond to Spot No in Table 1.