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In vitro myosin sliding filament assays. a) The mean velocities ± SD of at least 45 actin filaments during incubation with native myosin (gray bars) and myosin exchanged with noncaged semisynthetic (black bars) and caged semisynthetic (open bars) mRLCs. NMO, no motility observed; NonP, nonphosphorylated; P, phosphorylated by MLCK. b) Actin filament paths from a representative field before (−UV) and after (+UV) 90 s irradiation of myosin exchanged with cpSer19 mRLC 5.
