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. 2012 Jun 7;287(31):25706–25714. doi: 10.1074/jbc.M112.361360

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Klf4 was induced in the aorta of adenine-induced uremic rats. The calcification status was examined by von Kossa staining in the thoracic aorta of adenine-induced uremic rats (B and D) and controls (A and C). Expression of SM α-actin (E and F) and Klf4 (G and H) was examined by immunohistochemistry in the thoracic aorta of adenine-induced uremic rats (F and H) and controls (E and G). Representative pictures are shown from 4 mice analyzed per group. Bars: 1 mm (A and B); 50 μm (C–H). Inset in H is a magnified image from white lined square. Black and white arrowheads indicate Klf4-positive and Klf4-negative cells, respectively.