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. 2012 May 31;287(31):25795–25807. doi: 10.1074/jbc.M112.339622

FIGURE 1.

FIGURE 1.

Clonogenic mesenchymal stem and progenitor cells lack expression of CD44 in mouse BM. A, one representative FACS profile shows analysis of CD44 expression in CD45−LIN−CD31− cells. The numbers in the panels are mean percentages of the CD44+/− cells, from 10 experiments. B, FACS analysis of expressions of SCA1, CD51, CD90.1, CD105, VCAM1/CD106, and PDGFRa/CD140a in CD45−LIN−CD44+/− cells. The numbers in the panels indicate mean percentages of the gated cells within CD45−LIN−CD31− cells. The data are from 3–10 experiments. C, limiting dilution of CFU-Fs in the CD44+/− cells. The cells were plated at densities of 10, 50, 100, 200 cells for the CD44− cells and 200, 500, 1000, and 2000 cells per well for the CD44+ cells in 96-well plates. The cell dose yielding 37.5% negative wells for CD44− cells was 167, indicated by a dashed line. The 95% confidence interval bands are shown as dotted lines. There were no CFU-Fs observed from the CD44+ cells at any of the doses. D, frequencies of CFU-Fs in the CD44+ and CD44− cells calculated by L-Calc (Stem Cell Technologies). Data were mean ± 95% confidence interval, from three experiments. nd, not detectable. E, morphology of Giemsa-stained CFU-Fs derived from the CD44− cells. F, Q-PCR analysis of expressions of MSC-associated genes. Data are for three independent sorting experiments. Each dot represents the mean of triplicate measurements in each experiment. MSC-associated genes include Fmod, Igf1, Nov, Nes, Col1a1, and Angptl1. The differences between the two cell types were compared by unpaired one-tailed t test. PI, propidium iodide.