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. 2012 May 31;287(31):25795–25807. doi: 10.1074/jbc.M112.339622

FIGURE 5.

FIGURE 5.

Acquisition of CD44 expression in CD45−LIN−CD44− MSCs and progenitor cells after culture. The freshly sorted CD44− cells from human and mouse BM were cultured, and the expanded cells were analyzed by FACS for CD44 expression. A, CD44 expression in the expanded mouse stromal cells after 8–10 days of culture. B, CD44 expressions in the cultured cells at later passages (passages 5–15) derived a single CD44− mouse cell clone. Red lines, expression of CD44; blue lines, isotype control stainings. C, CD44 expression in the cultured human BM stromal cells. The freshly sorted CD45−CD235−CD31−CD44− cells from BM of healthy humans were cultured for 14 days. A15, A16, and A17 indicate different donors. Red lines, expression of CD44; blue lines, isotype control stainings. D, -fold expansion of the cells generated from the freshly sorted CD44− cells during culture. A limited number (10–50 cells) of the sorted CD44− cells were plated in culture, the cells generated from the culture were counted, and -fold expansion was calculated. The data are mean ± S.E. (error bars) from three independent experiments on BM from three healthy volunteers. The x axis indicates the numbers of the passages (P).