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. 2012 May 31;287(31):25795–25807. doi: 10.1074/jbc.M112.339622

FIGURE 6.

FIGURE 6.

Culture-induced alteration of cell adhesion molecule expression in MSCs from human BM. The microarray data on the freshly sorted CD44− mesenchymal cells and on the culture-expanded MSCs (acquired CD44 expression) derived from the fresh CD44− cells of the same donors were analyzed by dCHIP software. The microarray experiments on the culture-expanded MSCs were performed at passage (P) 1–3. a and b indicate replicate samples at each passage (P1–P3) from the indicated donors (a15 or a17). Clustering shows the genes being up-regulated more than 3-fold in the cultured cells compared with the freshly sorted CD44− cells. Red, high expression; blue, low expression. The numbers in the heat map are expression values of each indicated gene, and the -fold changes in expression of the receptors were calculated based on the mean expression values from each cell type. The data are from 10 microarray platforms (six arrays of the cultured cells and four arrays of the fresh cells) and two sorting experiments on two healthy volunteers, normalized by RMAExpress software.