FIGURE 8.

HDAC3 is necessary for PCA. A, BALB/c mice were given an intradermal injection of DNP-specific IgE (0.5 μg/kg). On the same day, BALB/c mice were given an intravenous injection of scrambled (100 nm) or HDAC3 siRNA (100 nm). The next day, BALB/c mice were given an intravenous injection of PBS or DNP-HSA (250 μg/kg) along with 2% (v/v) Evans blue solution. One hour after injection of Evans blue solution, the dye was eluted from the ear in 700 μl of formamide at 63 °C. The absorbance was measured at 620 nm. Representative images from four animals of each experimental group are shown. *, p < 0.05; ***, p < 0.0005. B, ear tissue lysates prepared from BALB/c mouse of each experimental group were subjected to a β-hexosaminidase activity assay (left). Tissue lysates were prepared 1 h after injection of DNP-HSA. One hour after injection of DNP-HSA, serum of each BALB/c mouse was obtained and subjected to a histamine release assay (right). Each experimental group consists of four BALB/c mice. C, same as B except that Western blot analysis was performed. D, immunohistochemistry staining was performed using paraffin section prepared from ear tissue of a BALB/c mouse of each experimental group (magnification, ×400; Olympus).