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. 2012 Jun 8;287(31):25881–25892. doi: 10.1074/jbc.M112.359521

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — TRAF2 binds to RSK2. A, TRAF2, but not TRAF5, interacts with RSK2. TRAF2-FLAG or TRAF5-HA with RSK2-Xpress was co-transfected into HEK239 cells. At 36 h after transfection, TRAF2-FLAG or TRAF1-HA was immunoprecipitated with anti-FLAG or anti-HA. Western blotting (IB) was performed using the indicated antibodies. B and C, endogenous RSK2 or TRAF2 interacts with endogenous TRAF2 or RSK2 in HaCaT cells. Cell extracts were used for immunoprecipitation with a TRAF2 or RSK2 antibody and control IgG. The immunoprecipitated complex was detected by Western blotting with an RSK2 or TRAF2 antibody. D, shown is the effect of TNF and EGF treatment on RSK2 binding to TRAF2. HaCaT cell extracts were treated with TNF or EGF and harvested at 5 or 10 min and then was used for immunoprecipitation with a TRAF2 antibody or control IgG. The immunoprecipitated complex was detected by Western blotting with the indicated antibody.