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. 2012 Jun 5;287(31):25975–25984. doi: 10.1074/jbc.M112.372136

FIGURE 1.

FIGURE 1.

Secondary structure analysis by circular dichroism. a, far UV-circular dichroism spectra of native HuPrP(90–231), mutants in position 129 at 4 °C. The color coding for mutant identification is shown in the inset. b, thermal unfolding curves of 129-mutants monitored at 222 nm. The colors are as shown in Fig. 1a. The dashed spectrum shows the thermal unfolding curve of WT HuPrP(121–231). c, far UV-circular dichroism spectra of mutants in position 129 at 4 °C before (solid line) and after (dashed line) one thermal unfolding/refolding.