FIGURE 2.

Influence of Lgt1 upon protein synthesis in S. cerevisiae translation extracts. A, synthesis of luciferase in yeast translation extracts in the absence (bars 1 and 5) or presence of 9 nm (bar 2), 90 nm (bar 3), and 280 nm (bar 4) Lgt1. Bar 5, experiment without luciferase-coding mRNA (i.e. no translation). Each bar represents means of two experiments ± S.D. Data are shown as percentage of maximal translation without added Lgt1. B, modification of eEF1A in yeast translational extracts by Lgt1. The reaction conditions were exactly the same as for in vitro translation but with 10 μm UDP-[¹⁴C]glucose instead of unlabeled UDP-glucose. Subsequently, the reaction mix was subjected to SDS-PAGE and autoradiography. Lanes 1 and 2, 9 nm Lgt1; lanes 3 and 4, 90 nm Lgt1; lanes 5 and 6, 280 nm Lgt1. Lanes 1, 3, and 5, incubation time of 10 min; lane 2, 4, and 6, incubation time of 60 min.