Toxicity of Lgt1 in wild type and Δhbs1 strains. Wild type and Δhbs1 S. cerevisiae, containing pESC-Ura or lgt1-D246N or lgt1-D246N/W520A within pESC-Ura-based plasmids, were analyzed on SD (left panel, glucose, Glc) or SGal (right panel, galactose, Gal). Description of strains is indicated on the left.