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. 2012 Jun 14;287(31):26052–26059. doi: 10.1074/jbc.M112.357590

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — EmrE(C_in) and EmrE(C_out) homodimers reassemble into a mixture of EmrE(C_in)/EmrE(C_out) heterodimers, EmrE(C_in) homodimers, and EmrE(C_out) homodimers after heating to 60 °C and cooling. A, BN-PAGE of cells expressing EmrE(C_in) and cells expressing EmrE(C_out) that were mixed before lysis (lane 3) or mixed after membrane collection but before solubilization in DDM (lane 4). EmrE(C_in) (lane 1), co-expressed EmrE(C_in)/EmrE(C_out) (lane 2), co-expressed EmrE(C_out)/EmrE(C_in) (lane 5), and EmrE(C_out) (lane 6) are included for comparison. B, BN-PAGE of DDM-solubilized EmrE(C_in) mixed with DDM-solubilized EmrE(C_out) and incubated at either 4 °C or 60 °C for different times as indicated (lanes 3–6). EmrE(C_in) (lane 1), co-expressed EmrE(C_in)/EmrE(C_out) (lane 2), and EmrE(C_out) (lane 7) are included for comparison. C, BN-PAGE of DDM-solubilized EmrE(C_in) mixed with DDM-solubilized EmrE(WT)-Myc-His (lanes 1–4) and of EmrE(C_out) mixed with DDM-solubilized EmrE(WT)-Myc-His (lanes 5–8) incubated at either 4 or 60 °C for different times as indicated. The composition of the different dimers are indicated by the same symbols as described in the legend to Fig. 2.