FIGURE 2.

mRNA and protein expression of FGFR1, FGFR3, and β-klotho in the mouse liver and fetal metatarsal chondrocytes. A, FGFR1, FGFR3, and β-klotho mRNA expression in chondrocytes and liver was assessed by real time PCR. Total RNA was extracted from chondrocytes isolated from fetal (20 days post-coitus) mouse metatarsal growth plates and from the liver of 3-week-old mice. It was then processed as described under “Materials and Methods.” The mRNA expression of FGFR1, FGFR3, and β-klotho mRNA was normalized by β-actin in the same samples. B, protein extracted from liver and chondrocytes was immunoblotted for FGFR1, FGFR3, and β-klotho. β-Actin was used as a loading control. A representative blot from three independent experiments is presented.