FIGURE 2.

Effect of silver nitrateon ethylene growth responses of dark-grown Arabidopsis seedlings. In all panels, seedlings were grown in darkness for 4 days under the indicated conditions. A concentration of 100 μl/liter ethylene and 100 μm silver nitrate was used. Data represent the mean hypocotyl length ± S.E. Differences between air and ethylene in the presence of silver were analyzed with t tests and considered statistically significant with p < 0.001 (*). A, hypocotyl growth of ethylene receptor loss-of-function and rte1 mutant seedlings were examined. Wild-type seedlings were included as controls. The etr1-7, etr2-3, ein4-4, etr2-3;ein4-4, etr1-6;etr2-3;ein4-4, and etr2-3;ein4-4;ers2-3, and rte1-2 mutants are in the Col background while etr1-9, ers1-3, and ers2-3 are in the Ws background. B, Col and etr1-7 seedlings treated with air or ethylene in the presence or absence of silver as labeled. Scale bar equals 2 mm. C, growth of etr1-6;etr2-3;ein4-4 triple mutants transformed with a wild-type genomic ETR1 (gETR1) transgene or a mutant transgene that lacks the conserved aspartate in the receiver domain for phosphotransfer (getr1 [D]). Col, Columbia; Ws, Wassilewskija.