FIGURE 1.
Localization of the Na,K-ATPase β2 subunit in nerve sections is similar to that of the Na,K-ATPase α2 subunit, but not of the Na,K-ATPase α1 subunit or β1 subunit. A, frozen sections of rat sciatic nerve were double stained by using mouse antibodies against α1 subunit (green) and either rabbit antibodies against α2 subunit (red) or rabbit antibodies against β2 subunit (red). B, frozen sections of human trigeminal nerve were double-stained by using either mouse antibodies against α1 subunit (green) or mouse antibodies against β1 subunit (green) and rabbit antibodies against β2 subunit (red). Anti-mouse Alexa Fluor 488-conjugated secondary antibodies were used to detect anti-α1 and anti-β1 primary antibodies, and anti-rabbit Alexa Fluor 633-conjugated secondary antibodies were used to detect anti-β2 and anti-α2 primary antibodies.