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FADD regulates cPKC activity and signaling. A, Western blot analysis of phosphorylated MARCKS in FADD+/+ and FADD−/− MEFs. MEFs were treated with 100 ng/ml PMA for a 30-min pretreatment with 5 μm Go6976 or not. B, Western blot analysis of phospho-(Ser) PKC substrate in FADD+/+ and FADD−/− MEFs. MEFs were treated with 100 ng/ml PMA for 1 h with or without pretreatment with 5 μm Go6976.
