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. 2012 Jun 13;287(31):26168–26176. doi: 10.1074/jbc.M112.378794

FIGURE 5.

FIGURE 5.

Non-isotype-specific inhibition of PKC with 42 μm rottlerin or 2.5 μm bisindolylmaleimide I decreased phosphorylation (P) at AS Ser-328. BAECs were pretreated with 42 μm rottlerin (Rott) or 2.5 μm bisindolylmaleimide I (Bis) for 30 min prior to stimulation with calcium ionophore (A23187) for 20 min. A, representative Western blot of three experiments. B, densitometric values represent the percentage of change from nontreated (NT) controls normalized to total eNOS or AS, respectively. Densitometric changes are significant with S.E. represented by error bars (n = 3, p < 0.05). C, culture medium from treated BAECs was assayed for nitrite with the DAN assay. A23187 significantly increased NO production with or without bisindolylmaleimide I (significance relative to nontreated: *, n = 3, p < 0.05; significance relative to A23187: ‡, n = 3, p < 0.05).