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PKCα, but not Akt, phosphorylate AS in vitro. A, in vitro kinase assays. Gray bars represent the incubation of purified AS minus kinase (Blank). Black bars represent the incubation of purified AS with the indicated kinase (Kinase). Kinase activity is represented as cpm with error bars representing S.E. (n = 3, *, p < 0.05). B, representative autoradiograph of in vitro kinase reactions separated by SDS-PAGE as described in A.
