FIGURE 4.

Rhinocetin binds to integrin α2β1. A, the binding of rhinocetin on the surface of platelets was assessed using an antibody raised against the snaclecs of Echis ocellatus by flow cytometry. The effect of various concentrations of rhinocetin on the binding of monoclonal antibody against α2 domain of integrin α2β1 (B) and GPIbα (C) was also assessed by flow cytometry. The level of antibody binding with vehicle was taken as 100%. Data represent mean ± S.D. (n = 3). D, 100 μg/ml of rhinocetin was incubated with platelets before lysing, and immunoprecipitation with antibody was raised against the snaclecs of E. ocellatus. The immunoprecipitated samples were run on 10% SDS-PAGE and probed with an antibody against α2 domain of integrin α2β1. The blot is representative of three different experiments. ***, p < 0.001 as calculated by t test. MW, molecular weight.