FIGURE 5.

Rhinocetin inhibits collagen-mediated inside-out platelet signaling. A, the effect of various concentrations of rhinocetin on fibrinogen binding upon 1 μg/ml collagen activation was analyzed by flow cytometry. R represents the fibrinogen binding in resting platelets. B, the effect of 100 μg/ml rhinocetin on fibrinogen binding was assessed with increasing concentrations of collagen (1 μg/ml, 5 μg/ml, 10 μg/ml) stimulation. C, the effect of 100 μg/ml rhinocetin on fibrinogen binding upon activation with 1 μg/ml of CRP-XL was assessed. The level of fibrinogen binding obtained with vehicle was taken as 100%. Data represent mean ± S.D. (n = 3). **, p < 0.01; ***, p < 0.001 as calculated by t test.