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. 2012 Jun 11;287(31):26235–26244. doi: 10.1074/jbc.M112.381483

FIGURE 6.

FIGURE 6.

Rhinocetin inhibits calcium mobilization and granule secretion. Calcium mobilization was measured in Fluo4 NW dye loaded platelets by spectrofluorimetry in the presence or absence of 100 μg/ml rhinocetin. Platelets were activated with 1 μg/ml of collagen (A), and the percentage of inhibition was shown (B). Data represent mean ± S.D. (n = 3). Platelets were stimulated with 1 μg/ml of collagen in the presence and absence of different concentrations of rhinocetin, and the level of P-selectin exposure was measured by flow cytometry (C). Data represent mean ± S.D. (n = 3). The level of P-selectin exposure with vehicle was taken as 100%. R represents the amount of P-selectin exposure in resting platelets. The level of ATP release upon 1 μg/ml of collagen activation in the presence or absence of different concentrations of rhinocetin at 90 s was measured by luminescence aggregometer (D). The level of ATP release obtained with vehicle was taken as 100% (E). Data represent mean ± S.D. (n = 3). **, p < 0.01; ***, p < 0.001 as calculated by t test. AU, arbitrary units.