FIGURE 7.

Rhinocetin limits thrombus formation in vitro. DiOC₆-labeled human blood was preincubated with vehicle or 25 μg/ml of rhinocetin and perfused over a collagen-coated Vena8 BioChip. Images were obtained every 30 s for up to 10 min, and representative images are shown in A and B. The volume (C) and fluorescence intensity (D) of thrombi obtained in presence of rhinocetin were compared with vehicle. The fluorescence (Fl.) intensity obtained at 10 min with vehicle was taken as 100% to calculate the % inhibition of rhinocetin on thrombus formation. Data represent mean ± S.D. (n = 3). ***, p < 0.001 as calculated by t test. AU, arbitrary units; DiOC₆, 3,3′-dihexyloxacarbocyanine iodide.