FIGURE 8.

Rhinocetin inhibits collagen induced proliferation and migration of HUVECs. Cells were cultured on 100 μg/ml collagen-coated wells before treating with vehicle or rhinocetin (100 μg/ml) and monitored under a light microscope for a period of 36 h after treatment with vehicle or rhinocetin. Rhinocetin did not show toxic effects to the cells (A) as the cell viability was maintained throughout. The images are representative of three independent experiments. The effect of rhinocetin on cell attachment on collagen surface (B), proliferation (C), and migration (D) were monitored and compared with vehicle. Data represent mean ± S.D. (n = three separate experiments and for each three different fields were measured). **, p < 0.01; ***, p < 0.001 as calculated by t test.