FIGURE 6.

HULC promotes proliferation of hepatoma cells through down-regulating p18 in vitro and in vivo. A, the proliferation of L-O2 cells treated with si-p18 was measured by MTT assay, and the protein levels of p18 were confirmed by Western blot analysis. OD Value, optical density value. B, cell proliferation of HepG2 cells co-transfected with si-HULC and si-p18 was determined by MTT assay. C, the average weight of tumors derived from HepG2 cells transfected with 100 nm si-HULC or/and si-p18. D, the growth curves of tumors. E, the image of tumors from nude mice. F, immunohistochemical staining for Ki-67 and BrdU in the tumor tissues from mice. Data are reported as mean ± S.D. for three independent experiments (**, p < 0.01, ***, p < 0.001, Student's t test).