TABLE 1.
Characterization of B-domain FV derivatives
ND, not determined; NA, not able to determine an accurate value.
| Cofactor species | Specific activitya | Specific activityb (+thrombin) | Kdc |
|---|---|---|---|
| Units/nmol | Units/nmol | nm | |
| FV-810 | 265 ± 49 | 308 ± 41 | 1.1 ± 0.3 |
| rFVa | 264 ± 30 | ND | 1.8 ± 0.2 |
| PD-FV | 27 ± 2 | 214 ± 9 | NA |
| FV-B226 | 29 ± 2 | 382 ± 29 | NA |
| FV-B208 | 4 ± 1 | 271 ± 20 | NA |
| FV-B199 | 9 ± 1 | 339 ± 74 | NA |
| FV-B152 | 223 ± 11 | 286 ± 43 | 2.6 ± 0.4 |
| FV-B104 | 13 ± 2 | 311 ± 33 | NA |
| FV-B-Ptex | 212 ± 49 | 308 ± 41 | 1.5 ± 0.2 |
| FV-B-Fish | 223 ± 39 | 245 ± 43 | 1.8 ± 0.8 |
| FV-B-Ch | 192 ± 31 | 327 ± 62 | 1.9 ± 0.3 |
| FV-B-Ch+hBR | 60 ± 13 | 273 ± 49 | NA |
| FV-B8-BR1 | 260 ± 16 | 293 ± 18 | ND |
| FV-B8-BR2 | 152 ± 15 | 268 ± 39 | ND |
| FV-B8-BR3 | 76 ± 13 | 232 ± 11 | ND |
a The specific clotting activity was determined as described under “Experimental Procedures.” The mean values ± S.D. of at least three determinations are presented.
b FV variants were incubated with thrombin before analysis.
c Data for binding studies can be found in Fig. 4 and supplemental Figs. S1 and S3.