Inactivating Gsα mutation reduces adipogenic tissues in vivo. (A): Adipose tissues from subcutaneous fat pads, abdominal white fat, and interscapular brown fat from Gsα+/p− (n = 3) and WT (n = 3) mice (left panels) were examined histologically with hematoxylin and eosin staining (right panels). (Supporting Information Fig. 2). (B): Expression of adipose markers was quantified using reverse-transcriptase polymerase chain reaction. *, p < .05; **, p < .01. Three independent experiments used cells from three WT and three mutant mice (analyzed individually in triplicate) for each experiment. Abbreviations: aP2, adipocyte protein 2; PPAR, peroxisome proliferator-activated receptor; UCP1, uncoupling protein 1; WT, wild type.