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. Author manuscript; available in PMC: 2013 Aug 31.
Published in final edited form as: J Immunol Methods. 2012 May 22;382(1-2):117–128. doi: 10.1016/j.jim.2012.05.011

Figure 7. Positivity criteria for IL-2.

Figure 7

A. The minimum analyte concentration to be considered positive was determined by assessing the background-subtracted IL-2 concentration measured in supernatant samples from Env-stimulated PBMC collected from vaccine participants before (Baseline) and after vaccination (Post-Immunization). Based on these measurements, a minimum analyte concentration threshold at 40pg/mL was selected for IL-2 (gray line). B. Fold over background IL-2 concentration measured in samples from Env-stimulated PBMC collected from vaccine participants before (Baseline) and after vaccination (Post-Immunization). A minimum 3-fold increase over background concentration was selected (gray line). Similar criteria were determined for all analytes.