. Author manuscript; available in PMC: 2013 Aug 31.

Published in final edited form as: J Immunol Methods. 2012 May 22;382(1-2):117–128. doi: 10.1016/j.jim.2012.05.011

Table 2.

Measurement of known concentrations of IL-2 and TNF-α by multiplex bead array and by ELISA.

Analyte	Expected Concentration (pg/mL)	Assay	Mean Concentration^* (pg/mL)
IL-2	10	MBA ELISA	5.5 6.0
	100	MBA ELISA	35.1 39.6
	1000	MBA ELISA	461.4 517.7
TNF-α	5	MBA ELISA	1 2.4
	50	MBA ELISA	7.2 16.9
	500	MBA ELISA	94.1 192.4

Note that samples were plated in duplicate.