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. 2012 May 22;21(16):3604–3618. doi: 10.1093/hmg/dds191

Figure 6.

Figure 6.

Cytokinesis defects in cells from Spg20−/− mice. (A) Representative MEFs from Spg20+/+ and Spg20−/− mice stained with β-tubulin (white) and DAPI (blue). The merged image is shown, and an arrow identifies a binucleated cell. Individual channels are shown as insets. Scale bar, 10 µm. (B) Quantification of multinucleated MEFs in Spg20+/+ and Spg20−/− mice. **P < 0.01. (C) Left, Representative trajectories of individual primary Spg20+/+ and Spg20−/− MEFs from the frame-by-frame analysis of the time-lapse recordings during a 14-hour observation period. Vertical and horizontal scale bars, 50 µm. Right, Migration velocities of the indicated MEFs are graphed (means ± SD; n = 20). Migration data comprise at least 10 cells from 3 independent Spg20+/+ and Spg20−/− pairs. (D) Time-lapse DIC images from 200 min of analysis, with times indicated. Arrowheads indicate dividing cells. Scale bar, 10 µm. (E) Whole mount Alcian Blue/Alizarin Red staining of Spg20+/+ and Spg20−/− mouse skeletons at P1. Scale bar, 1 cm. (F) H&E-stained sections of the proliferative zone of the tibial epiphyseal growth plate of Spg20+/+ and Spg20−/− mice. Scale bar, 500 µm. (G) Left, H&E-stained sections of the knee joint region of P28 Spg20+/+ and Spg20−/− mice. The indicated areas are enlarged in the insets, demonstrating multiple binucleated chondrocytes. Right, Quantification of binucleated cells in growth plates of P28 Spg20+/+ and Spg20−/− mice (means ± SD; n = 3 trials, with 100 cells per trial for each genotype). ***P < 0.005. Scale bar, 100 µm.