Table 2.

Summary information for the three candidate genes and 76 EST markers

Maker name	Type of marker	SNP region	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Product length (bp)	Annealing temp. (°C)	Restriction enzyme
FeCOL3	CAPS	intron	TCAAGACTCAGCTGGTGAACG	GGACGGATCAGAAAACTCGTC	713	62	BclI
FeCCA1	CAPS	exon	TCGAGCCAAGCAGTTACTACG	CCACAAGAGGAACGGTCAAC	901	62	Hpy188I
FeELF3b	CAPS	intron	TTGGGAGTTCTGGGGATGAG	TGCCTTGCTCTTTTCCTGGT	1280	62	DraI
Fest_F0005b	EST	–	CAAGCCAACAAGCTGGAGAA	AATGGGAGAATGCTTAGTTGCTTAC	810	62	–
Fest_F0035	EST	–	CAGCTAAGGCAGACGGTTGA	TCATTGCCAGATCTCATTGGA	148	58	–
Fest_F0049	EST	–	GTGAAATTATGTTTTCTTTTAGAGG	TTCTTGTCTATGAGCACTGAAA	123	54	–
Fest_F0052a	EST	–	GGACGGACATTGGCTGATTAC	TGACCAACCACACACAAAACAC	163	58	–
Fest_F0056a	EST	–	AAGGATCACAAGAAACCGGAA	CAAGCCAAGTATCCATGACAAAC	266	62	–
Fest_F0057	EST	–	TCTTTGTTGTGTCCAAGATTGCTG	CCAAATCGTAATCATAAGCGTTCC	314	54	–
Fest_F0078	EST	–	AAAACCCCTCTTTTCTCCCC	TGGCCTTGATTCCACCTTCT	217	62	–
Fest_F0090	EST	–	CAGCAGTTCATGCATCTGGTAA	CGATGATGATGATGGTGGAGA	348	58	–
Fest_F0095	EST	–	ATTACTGCCACCTCATCAGACC	AGATCGCTCAGTATCGTGCC	699	62	–
Fest_F0110	EST	–	GAGGAGGAACAAGACGCACA	TCAAGCCAGTGACATACCACAA	1354	62	–
Fest_F0117	EST	–	GAGTCCTTTTCCAAGCCCATC	TGGAGAGGTCAATTCCAGCC	702	58	–
Fest_F0119b	EST	–	GGGAGGATTCATTTCTACAGCA	CCAATGTCAGCAGCTTAAGAGAG	183	58	–
Fest_F0129	EST	–	TGGACGAGCTTTCATAGACG	TCTTCTGAAACATAAATCACAAATG	161	54	–
Fest_F0138b	EST	–	CACATACCAACCAGAACTCAATACA	GCCCGAGTATCGTTTGCTCTC	184	58	–
Fest_L0080	EST	–	CTTCAGTTTTCCAGTTCCTTCCA	CAGCGCAATGTTTCCCTTC	787	54	–
Fest_L0433	EST	–	GAAAGAGTTCAAACAAGAGC	AACACCACTCTTCAAAGTTC	1128	58	–
Fest_F0031_1	CAPS	exon	CAAAGCACGTCCAAACAACA	GGATAATGGCGGTGTCAAAA	788	58	Hpy188I
Fest_F0053_2	CAPS	exon	GCGTTATTTCCCGACTGATG	GATGCACATAGACATGGCTGG	212	62	BsuRI
Fest_F0076_9a	CAPS	exon	TTCTGTTAGTTAAAATGAAAATACA	ATAGCAACGGACACAAATAA	613	54	HindIII
Fest_F0077_2	CAPS	exon	TTCGGGAGAAATCACAAATACG	TCGAAAGGATTGTTTGCAGTTG	498	62	ApaLI
Fest_F0081_7	CAPS	exon	CATTACAACACACGCATCGG	AGCAAGGCAAGCTCTTCTGG	369	58	BshNI
Fest_F0083_8	CAPS	intron	CATGCGCAAACTCCCTTTC	CGTACCACGATCAATTTACAGATCA	2010	54	MspI
Fest_F0087_2	CAPS	exon	CAGACCTCAAATGTTCCACCAG	GAAGAGGCAAAGGAGGCAAG	520	62	TaqI
Fest_F0100_15a	CAPS	intron	TCCAATCGCGTTGACAGAA	GAAAATGCCGTCCCACAAG	431	58	AvaII
Fest_F0102_1a	CAPS	exon	GGGCAGACTCGGTGCTATTC	GGTGAAGGGATTGTGGCTGT	291	62	HinfI
Fest_F0139_3	CAPS	exon	ATTCTGGTGTTCACTGCTCTTA	GAGGCCATTGATCGTGATTC	357	62	TaqI
Fest_L0030_1	CAPS	intron	AAAGAAGGAAAAGGCTCCCC	GATGCAACTATCCATGCCCC	1135	58	BsuRI
Fest_L0041_2	CAPS	exon	GTCCACAGGAGGAAAGGCAC	CGTGAAACACCAAATTACGACC	527	62	TaqI
Fest_L0046_2	CAPS	exon	CATGTTCTCAAGCAGCACAAAA	GGATACAGGGTTGGTGGAGG	373	62	HinfI
Fest_L0064_2	CAPS	exon	CGGAGAATGGCTTCCAAGAG	GAGAAGTGGCATCTGGCTGG	659	66	TaqI
Fest_L0101_1	CAPS	intron	GGCATCCATGTTTAGCTCTGG	ACCTCAAGGGCACGGTTCT	392	62	HinfI
Fest_L0124_7	CAPS	exon	CCACCTGGTCCTTCGTCTTC	GAAACGCCACCAACCATACC	376	66	MspI
Fest_L0130_2	CAPS	exon	CTGAACCAACACAAACGATCAA	TGGTTTGGAACAAGGAAGTCG	1386	62	HinfI
Fest_L0136_5	CAPS	exon	AAGCAGCACCTTCACAGCAA	CGAAGAAGCTGGGGTCGTAG	431	66	BsuRI
Fest_L0169_3	CAPS	exon	AACCTCGCATTTTCAGTCCC	ACAGCAATACTCCGGGCTTC	629	60	MspI
Fest_L0186_3	CAPS	exon	CCACACACACCAAAAGCACC	CACAGAACTAGGACGACTCCTGAA	492	62	TaqI
Fest_L0211_5b	CAPS	exon	GGGAATCGGAGTTGGTTACTCTC	CTGGTGGCTTTGGATGGTTT	525	62	TaqI
Fest_L0219_2	CAPS	exon	GCTTGTAGATGGGGTGTTGG	CGTTTCGGCAGAGTTTCATC	717	62	StyI
Fest_L0266_5	CAPS	exon	TCTCTCATCCCAAAACCCAA	TAATCCAACGGCTCAAACCA	391	60	HhaI
Fest_L0292_1	CAPS	exon	ACCCGTCCCAGTCAAAGAAA	AAAGTCATAAACCCTGATGCCC	479	60	BsoBI
Fest_L0377_4	CAPS	exon	CGTTTTCATGGTGTCGTTCC	CGGAGAAACGGGTAAGGTGT	576	62	EcoRV
Fest_L0426_12	CAPS	intron	ATCGATTTCGAGGGGTTTTG	TATTGAGGAGGCCCATTCTG	700	62	MseI
Fest_L0432_1	CAPS	exon	CAAAGCAGGCAGAGAAAGCA	GCAGATGCAAAATCAACTTCCC	559	62	AvaII
Fest_L0480_1	CAPS	intron	TGCTGCAACTCACGAGATTAAC	TGGATGCCAGTTCAAAGGTC	597	62	BglII
Fest_L0490_2	CAPS	exon	GAGCAGCGGGTTGTCTTCT	TAGCGTCTCCAAACTGTCCG	808	62	HhaI
Fest_L0494_6	CAPS	exon	TCCACAATCTCTCCTTCCTCC	AGCACCAACAAACGCAAAAC	603	62	TaqI
Fest_L0506_1	CAPS	intron	CGTCGTCATTCTCGCTCTCC	GCATTCATCATTGGGGCTGT	870	62	DraI
Fest_L0542_3a	CAPS	exon	GCGAATTCGGTTTCACAAAA	TACCCTCGCCGACTACAACA	427	62	KpnI
Fest_L0543_1	CAPS	exon	TTGATTCCTGGGCAACAGAG	ACTTGACAGCAATGCAAAACG	488	60	MspI
Fest_L0556_6	CAPS	exon	CCCTAATAACCCCGAAACCC	AAAGCCTTCTCAGCAGTGTCC	481	62	MspI
Fest_L0578_2	CAPS	exon	GGCCCGTCAAAACCAAAA	CCCTGAACCTCAATTGCGAC	375	62	SacI
Fest_L0606_4	CAPS	exon	TGACAAGATGAAGGAGCTGGA	AGCAGGAGAGCCTGTTGATTT	308	62	TaqI
Fest_L0609_1a	CAPS	exon	TGCCTCTAACTTGTTCCAATTCC	TCGTCGACATCATCTTCCGT	227	62	AseI
Fest_L0706_2	CAPS	exon	GGGGAGTGAGGAAACCAACA	ACAGAAACCCAATCAATCCACC	514	62	BstXI
Fest_L0712_10	CAPS	exon	TAACAACCCGCTCTCTTCCC	ATCCATGACACGTCTCGCTC	592	62	HinfI
Fest_F0032_2	dCAPS	intron	ACGCATTCAAATTCAATGCA	GAAGAAGCTGGCGAAGAAGA	154	52	EcoT22I
Fest_F0034_1	dCAPS	intron	TACTTGATCCAAATCCTTAATATTAGGATC	CGGGATTCTTTGGTGGTTT	151	58	BamHI
Fest_F0043_1	dCAPS	exon	TACCGGCTTCCCATTGTCAA	TTCCGGAAAGAGGAATCACC	180	62	HindII
Fest_F0050_3	dCAPS	exon	GTGTCCTCGAAAAGTCCACC	GCCCTTAGGGAGATCAGGAA	182	60	StyI
Fest_F0075_2	dCAPS	exon	AGAAGTGAGGATGACTTTAA	ATTTCCCATTGGCTTTTT	158	52	DraI
Fest_F0079_1	dCAPS	exon	TCGATTTTTACCTTCACCCGCCATGGTCAA	CAGTTTAACGGCAAACATACCCTTTT	138	56	HindII
Fest_F0080_4	dCAPS	exon	TGAGGAGGTGAGGCTTACCG	TGTTTTGTGGGTTTTTTGACTG	158	58	HhaI
Fest_F0085_1a	dCAPS	exon	GACCAAAGAAACAAATTTTA	TTGGTTGGTCATTAATCTAT	151	52	DraI
Fest_F0101_1	dCAPS	exon	AGGATAAAAAGAAGAAGAAGAGTAAAGATT	CCTGCGACATGCATAATTAAA	176	58	HinfI
Fest_F0108_1	dCAPS	exon	GAAGTAGTTGTAGACAGAGT	TAGTTTGGTAATAATCTAGC	224	50	HinfI
Fest_F0112_3	dCAPS	exon	TACTAATACAAACTTAATTT	CTTATGATAGGTGCTAAT	198	42	DraI
Fest_F0127_1	dCAPS	exon	CGCCGTCGCCGCCGCTCAGGAGTCTCCGTC	TCCAATCGCTCAGAAGAGGAATCCGAAGG	139	62	HindII
Fest_F0133_1	dCAPS	exon	AAGTGTCCTTGATCTTCAAG	AGCTCAACAACTCTTCACTT	175	50	HindIII
Fest_F0134_1	dCAPS	exon	CAAGAACCTCCAAAGTAAGTTCTAATATGC	GCTTACAATTGCAACTTTACAACGA	168	58	EcoT22I
Fest_L0053_3	dCAPS	exon	AACTACAGTACTGGTGGGTC	CTCATCACCCTTCTTCTTC	164	54	MspI
Fest_L0129_12a	dCAPS	exon	GCGCAGCTTGCTATTGCTCC	CCCAAAGATCCCAATCCCTC	177	56	MspI
Fest_L0242_1	dCAPS	exon	CGCTATGCCGCCTTACTGCG	GCCATGTCGCGGAAGAAGGTC	167	54	HhaI
Fest_L0329_3	dCAPS	intron	CATCCATTCTTGATTTCAAG	TACACTCAGGAGTCAGGAAC	171	56	ApoI
Fest_L0337_6	dCAPS	exon	ATCTTCAGGCCTAATGTTAA	AATCAGCAAACTCTCTAAGC	289	54	HindII
Fest_L0472_1	dCAPS	exon	GGATGAGGTGGGGCCAGGTGCCAGAGCTGT	TTCAAGGGAGAAATGGAGGCCATGA	123	56	BstXI
Fest_L0582_7	dCAPS	exon	GAAAACATGTTCTTAGATAT	AGAAGGATAGCTTAAATG	154	50	EcoRV

^aMarker showed segregation ratio that was significantly different from the expected Mendelian ratio.

^bMarker could not be mapped (Fig. 2).