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. 2011 Dec 15;61(4):327–337. doi: 10.1270/jsbbs.61.327

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Copyright © 2011 by JAPANESE SOCIETY OF BREEDING

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 7 — Hybridity and appearance of the parents and their hybrids (BC₁ plants). GISH analysis of a triploid progeny with 24 L. × formolongi (green) and 12 L. rubellum (dark blue) chromosomes (A). CAPS analysis of MspI-digestion of the rDNA ITS region (B): 1, HincII-digested ϕX174 markers; 2, L. × formolongi; 3, ‘Otome no Sugata’; 4, L. rubellum; and 5, a hybrid. The appearance of a female parent (L. × formolongi) (C), male parent (‘Otome no Sugata’) (D) and BC₁ shoots (E–H). Bars = 10 μm (A) or 10 cm (C–H).