Table 1.
Marker haplotypes of the wheat-D. breviaristatum additionsa
| Deletion binb | Markers | Y93-1-6-6 | Y93-1-A6-4 | Homologous relationship | Enzyme |
|---|---|---|---|---|---|
| C-2BL2-0.36 | BE517627-STS | −c | + | 2L | Hae III |
| C-2BL2-0.36 | BE443711-STS | − | + | 2L | HaeIII |
| C-2BL2-0.36 | BE591670-STS | − | + | 2L | RsaI |
| 2BL6-0.89-1.00 | BE444894-STS | − | + | 2L | AluI |
| 2AL3-0.77-1.00; | |||||
| 2BL6-0.89-1.00; | TNAC1142-STS | − | + | 2L | d |
| 2DL6-0.94-1.00 | |||||
| C-7DL5-0.30 | BE498418-STS | − | + | 7L | AluI |
| C-7DL5-0.30 | BE498849-STS | − | + | 7L | RsaI |
| C-7DL5-0.30; | BF483007-STS | − | + | 7L | d |
| 7AL1-0.39-0.63; | |||||
| 7BL2-0.38-0.63; | TNAC1815-STS | − | + | 7L | TaqI |
| 7DL5-0.30-0.61 | |||||
| 7AL16-0.86-0.90; | |||||
| 7BL6-0.84-0.86; | TNAC1826-STS | − | + | 7L | TaqI |
| 7DL3-0.82-1.00 | |||||
| 7AL21-0.74-0.86 | BE442572-STS | − | + | 7L | RsaI |
| 7DL3-0.82-1.00 | BF294002-STS | − | + | 7L | HaeIII/RsaI |
| 7AL16-0.86-0.90 | BE518357-STS | − | + | 7L | b |
| 7DL3-0.82-1.00 | BE637838-STS | − | + | 7L | MspI |
| 7DL3-0.82-1.00 | BE404744-STS | − | + | 7L | MspI/RsaI |
| 7DL3-0.82-1.00 | BG314261-STS | − | + | 7L | HaeIII/AluI |
| 7DL3-0.82-1.00 | BG262960-STS | − | + | 7L | AluI |
| 1BL3-0.85-1.00 | BE446672-STS | − | + | 1L | HaeIII/MspI/RsaI |
| 1BL3-0.85-1.00 | BE591325-STS | − | + | 1L | RsaI |
| C-1BL6-0.32; | BE405613-STS | − | + | 1L | RsaI |
| 7AS2-0.73-0.83; | |||||
| 7BS2-0.27-1.00; | TNAC1782-STS | − | + | 7S | TaqI |
| 7DS4-0.73-1.00 | |||||
| 7AS1-0.89-1.00 | BF473884-STS | − | + | 7S | RsaI |
| 6AS1-0.35-0.65; | |||||
| 6BS4-0.46-0.76; | TNAC1683-STS | − | + | 6S | TaqI |
| 6DS1-0.54-0.79 | |||||
| C-6AS1-0.35 | BE445239-STS | − | + | 6S | MspI/RsaI |
| 6DS6-0.99-1.00 | BG262210-STS | − | + | 6S | MspI |
| C-6AL4-0.55; | |||||
| C-6BL3-0.36; | TNAC1702-STS | + | − | 6L | TaqI |
| 6DL6-0.29-0.47 | |||||
| C-6BL3-0.36 | BE498480-STS | + | − | 6L | RsaI |
| C-6BL3-0.36 | BE443951-STS | + | − | 6L | MspI/RsaI |
| 6AL8-0.90-1.00 | BE637963-STS | + | − | 6L | HaeIII |
| C-6BL3-0.36 | BE404912-STS | + | − | 6L | MspI |
| 6DS4-0.79-0.99 | BE591939-STS | + | − | 6S | MspI |
| C-7BS1-0.27 | BE404728-STS | + | − | 7S | RsaI |
| C-7DS5-0.36 | BE585744-STS | + | − | 7S | HaeIII/MspI/RsaI/AluI e |
| 7DS4-0.61-1.00 | BE606678-STS | + | − | 7S | HaeIII/RsaI |
| 7DS5-0.36-0.61 | BE489493-STS | + | − | 7S | HaeIII |
| 7DS5-0.36-0.61 | BG262748-STS | + | − | 7S | HaeIII/MspI/RsaI/AluI |
| 7DS4-0.61-1.00 | BE586111-STS | + | − | 7S | RsaI |
a
Polymorphism was obtained from PCR amplification (and sometimes also with PCR products digested by 4-base cutter enzymes) by all the primers listed in Table 1.
b
Consensus bin location of mapped ESTs taken from http://wheat.pw.usda.gov/cgi4bin/westsql/map_locus.cgi.
c
−, no polymorphism, +, polymorphism detected.
d
Enzyme digestion is not necessary for these primers.
e
Polymorphic PCR products following digestion by HaeIII, or MspI, or RsaI, or AluI indicated as Primer/HaeIII/MspI/RsaI/AluI.