Fig. 1.

Breeding scheme for the development of the CSSLs and progeny selection. To obtain the substituted segments on target chromosome, CSSL₅₀ was backcrossed to Asominori followed by self-pollination until BC₄F₁, and the genetic background was selected by SSR markers on target/non-target chromosome. The BC₄F₁ individuals, designated as CSSL50-1, harbored a small segment spanning R727–X397. Sub-CSSLs, the progenies of CSSL50-1, were employed for further study. AA type and II type, represent lines homozygous for Asominori (AA) and IR24 (II) alleles, respectively, were used to identify the phenotype. And IA type, represents lines heterozygous for Asominori and IR24 alleles, was used to further shorten the segments on target interval.