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. 2012 Feb 4;61(5):639–645. doi: 10.1270/jsbbs.61.639

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Copyright © 2012 by JAPANESE SOCIETY OF BREEDING

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Separation and detection of group A saponins, Ab and Af, by thin layer chromatography (TLC) and DDMP saponin βa by high performance liquid chromatography (HPLC). A: TLC patterns of aqueous 70% ethanol extracts of seed hypocotyls. Ab: Fukuyutaka (Ab phenotype, Sg-3), Af: Mikuriya-ao (Af phenotype, sg-3). Arrows indicate the positions of respective group A saponins. R_f: relative to front. B: HPLC chromatograms of the extracts. Upper: Ibarakimame 7 (βa presence, Sg-4), lower: Suzuyutaka (βa absence, sg-4).