Table 2.
Enhancement of disease resistance through transgene-induced RNA silencing targeted to pathogens in soybean
| Target gene | Construct | Promoter | Transformation method | Reference |
|---|---|---|---|---|
| Soybean mosaic virus, CP gene and 3′ UTR | cDNA | CaMV 35S | A. tumefaciensa | Wang et al. 2001 |
| Bean pod mottle virus, CP gene | cDNA | CaMV 35S | Particle bombardment | Reddy et al. 2001c |
| Soybean dwarf virus, CP gene | cDNA IR | CaMV 35S | Particle bombardment | Tougou et al. 2006 |
| Soybean dwarf virus, CP gene | cDNA | CaMV 35S | Particle bombardment | Tougou et al. 2007 |
| Soybean mosaic virus, CP gene | cDNA | CaMV 35S | Particle bombardment | Furutani et al. 2006, 2007 |
| Cyst nematode (Heterodera glycines), major sperm protein gene | cDNA IR | Arabidopsis ACT2 | Particle bombardment | Steeves et al. 2006 |
| H. glycines genes Cpn-1, Y25 and Prp-17 | cDNA IR | CaMV 35S | A. rhizogenesb | Li et al. 2010 |
| Root-knot nematode (Meloidogyne incognita) TP and MSP genes | cDNA IR | FMV | A. rhizogenesb | Ibrahim et al. 2011 |
a
Agrobacterium tumefaciens-mediated transformation.
b
Agrobacterium rhizogenes-mediated root transformation.
c
The mechanism of virus resistance in this report could be mainly brought about by the expressed CP protein rather than through RNA silencing. However, we could not exclude the possibility of the involvement of RNA silencing in the phenomenon because no data of the level of viral RNA or CP mRNA in the virus-infected plants is presented.
Abbreviations: CP, coat protein; IR, inverted repeat; CaMV, Cauliflower mosaic virus; ACT2, actin 2; FMV, Figwort mosaic virus; TP, tyrosine phosphatase; MSP, mitochondrial stress-70 protein precursor.