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Southern blot and PCR analysis of GmKASIIA and GmKASIIB genes in the normal soybean cultivar Bay and three high-palmitic-acid mutants. (A) Hind III-digested DNA fragments (lane 1, Bay; lane 2, KK7; lane 3, J10; lane 4, M22) were hybridized with a GmKASIIA cRNA probe. (B) GmKASIIA and GmKASIIB DNA fragments were amplified with gene-specific primer sets (lane 1, Bay; lane 2, KK7; lane 3, J10; lane 4, M22).
