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Development of PCR-based markers for the J10 and M22 mutations. (A) Locations of two primer sets for detecting the mutation in J10. N.D. means not detected. (B) Location of a primer set for detecting the mutation in M22. (C) Detection of the J10 mutation: Bay (lane 1), heterozygous (lane 2) and J10 (lane 3) DNA templates. (D) Detection of the M22 mutation: Bay (lane 1), heterozygous (lane 2) and M22 (lane 3) DNA templates.
