Table 2.
Nucleotide sequence and amplicon size of each primer used in this study
| Primers | Forward (5′-3′) | Reverse (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| For determination of deleted region in J10 | |||
| KASIIA-ORF | TTGCGAAGGGTCATATTAGTGCATC | TTAAGAACAAGCCTAACAGGCTGAC | 2109 |
| KASIIB-ORF | AATTTGCTTGATGGTGTTAGTGGCATAAGT | GGGTTGATGAACTAATGAACCTTCAG | 1376 |
| For examination of J10 deletion upstream (5′ end) of GmKASIIA) | |||
| −84 | CAAACGCTACGAGCTAGAGAGGTTG | CCACTTAGGCTTTGTGTTGTCTCCTT | 507 |
| −127 | TGGACCGATTCAACGACCAAGTCGAAC | TTACACGCGTCTTGCCGCATGCTCAA | 400 |
| −142 | GGAATGCTGCGACAAAACTCAACAC | GTGTACAACTGGCATGTTTGCGAAGA | 792 |
| −148 | TACAGTGCATCGAAATGAGCAACAC | AAGATCTGTTTCTCCGCATTGGACT | 651 |
| −154 | AATGGGACTTTGTGTTTCCAATCCC | GTGTCAGAAAATCTCTTCTCCAACTC | 187 |
| −169 | CCTTATCACGTGCGTATCCGACACT | ACTTGCCCAATCCAACTCTCAACAC | 992 |
| For examination of J10 deletion downstream (3′ end) of GmKASIIA) | |||
| +12 | ATTAACAAGTCCAACTATGACTCTT | TATATGAATGCTTTATCATTGCCAAC | 749 |
| +43 | TTGCTAATTTTCGTCACCATCGCAA | AGCCAACTTAACACATCATGCATCT | 720 |
| +53 | AATGCGAAGAACACCATAAAGTCCA | ACGATTCTGGAATACACCACTCTCT | 502 |
| +57 | TAGGCAAGCTATCTAAATCTCGTCA | TATCACTTATGGATTCCGTACCTCT | 794 |
| For identification of mis-spliced GmKASIIB transcripts | |||
| KASIIB-RT | TCTCGCAAGTCTAATTCAACGTC | GTTTTGACCAAAACAATGCATTAGAGCTT | 906 |
| For identification of mis-spliced GmKASIIB transcripts | |||
| JM (J10 marker) | CTTTGGAATGCAGTACGTGTCGGAT | CTCAGCGCAAATGTCATTACGGAGA | 207,301 (Bay) 1099 (J10) |
| MM (M22 marker) | TTACTCATTCTCTGTGGCTTGTGTG | GGGTTGATGAACTAATGAACCTTCAG | 254 (Bay) 228 (M22) |