Table 2.
Overview of the mutant resources used in the TILLING method in various plant species
| Mutagen | Mutation type | Plant species | Mutagen dose | Mutation frequency | Reference |
|---|---|---|---|---|---|
| Chemical agents | |||||
| EMS | Point mutations | Arabidopsis | 20–40 mM | 3.3/Mb | Greene et al. (2003) |
| EMS | Point mutations | Barley | 20–30 mM | 1.0/Mb | Caldwell et al. (2004) |
| EMS | Point mutations | Brassica napus | 24–48 mM | 7.6–24.1/Mb | Wang et al. (2008) |
| EMS | Point mutations | Brassica rapa | 24–32 mM | 16.7/Mb | Stephenson et al. (2010) |
| EMS | Point mutations | Maize | 5 mM* | 0.9–2.1/Mb | Weil and Morde (2007) |
| EMS | Point mutations | Medicago | 12 mM | 2.1/Mb | Le Signor et al. (2009) |
| EMS | Point mutations | Pea | 4 mM | 1.5/Mb | Triques et al. (2007) |
| EMS | Point mutations | Peanut | 30–96 mM | 0.9–1.1/Mb | Knoll et al. (2011) |
| EMS | Point mutations | Rice | 130 mM | 3.8/Mb | Till et al. (2007) |
| EMS | Point mutations | Sorghum | 8–24 mM | 1.9/Mb | Xin et al. (2008) |
| EMS | Point mutations | Soybean | 40–50 mM | 1.8–7.1/Mb | Cooper et al. (2008) |
| EMS | Point mutations | Soybean | 30–40 mM | 0.3–1.3/Mb | Present study |
| EMS | Point mutations | Tomato | 56–80 mM | 1.7–3.1/Mb | Minoia et al. (2010) |
| EMS | Point mutations | Wheat | 60–96 mM | 41.7/Mb | Slade et al. (2005) |
| EMS | Point mutations | Wheat (durum) | 60–80 mM | 25.0/Mb | Slade et al. (2005) |
| NMU | Point mutations | Rice | 1 mM* | 7.4/Mb | Suzuki et al. (2008) |
| NMU | Point mutations | Soybean | 2.5 mM | 7.1/Mb | Cooper et al. (2008) |
| NaN3 | Point mutations | Barley | 10 mM | 2.6/Mb | Talamè et al. (2008) |
|
| |||||
| Physical agents | |||||
| γ-rays | Deletions/point mutations | Rice | 500 Gy | 0.2/Mb | Sato et al. (2006) |
| X-rays | Deletions | Soybean | 200 Gy | 0.1/Mb | Present study |
EMS, ethylmethane sulfonate; NMU, N-methyl-N-nitrosourea
*
These mutant populations were developed from progeny pollinated with mutagen-treated pollen.