Fig. 3.

Superposition of aaNAT2, aaNAT5b, aaNAT7, and other representative GNAT structures and the phylogenetic tree. (A) Superposition of eight GNAT structures (PDB codes 1PUA, 1I21, 1S3Z, 1CJW, and 1M4G, as well as the three mosquito putative aaNATs) reveals that GNAT common fold core structures are nearly universally conserved despite structure-based sequence alignments that reveal very low pairwise sequence identity. Motifs C, D, A, and B are well superposed, labeled as C, D, A, and B, respectively. (B) The phylogenetic tree based on the 3D structural alignment of 15 GNAT structures, including aaNAT2, aaNAT5b, and paaNAT7. After 3D alignment, the “PhylogeneticTree_TreeTop” function in STRAP was used to draw a cluster algorithm-type phylogenetic tree.