Fig. 3.

Dynamical properties of the F-actin network: periodicity in F-actin production at the boundary and actin-based structure fusion. (A) Time series of F-actin production at the droplet boundary. (B) Kymograph representation and time evolution of the integrated intensity of a region of interest localized at the vicinity of the droplet boundary. F-actin production shows periodicity with a period of approximately 100 s. (C) Time series acquisitions of two droplets undergoing fusion. (D) Characterization of velocity distributions for three steps: (i) prior to droplet fusion, (ii) during F-actin network fusion, and (iii) during ring-like structure fusion. (E) Evolution of the mean velocity in a second fusion droplet event. Scale bars, 10 μm.