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. 2012 Jul 2;109(29):11782–11787. doi: 10.1073/pnas.1209292109

Fig. 5.

Fig. 5.

Characterization and properties HA22-LR-LO10. (A) SDS PAGE gel of purified RITs. The purified proteins (2 μg) were analyzed on a 4–20% gradient SDS polyacrylamide gel under nonreducing conditions (lanes 1 and 2) and under reducing conditions (lanes 4 and 5). Lanes 1 and 4, HA22-LR; lanes 2 and 5, HA22-LR-LO10; molecular mass standards are indicated on the left; gray arrows, VH-toxin; black arrow, VL. (B) Cytotoxic activity of HA22-LR and HA22-LR-LO10 on Daudi cells. The cytotoxicity assays were performed at least five times using three values for each point. ●, HA22-LR; ○, HA22-LR-LO10. Data are expressed as the mean ± SD. (C) Summary of cytotoxic activity of HA22-LR and HA22-LR-LO10 on various CD22+ cells. A431 cells are a CD22− control. (D) Antitumor activity. Groups of five SCID mice bearing CA46 tumors were treated every other day × 3 (arrows), with 0.2% mouse serum albumin in PBS (◆), HA22-LR at 1.0 mg/kg (▲), HA22-LR-LO10 at 1.0 mg/kg (△), 3.0 mg/kg (○), or 5 mg/kg (□). *P < 0.05.