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. 2012 Jul 2;109(29):11782–11787. doi: 10.1073/pnas.1209292109

Fig. 6.

Fig. 6.

The reactivity of HA22-LR-LO10 and various mutant RITs with human antisera. (A) A typical result showing displacement curves measuring the reactivity of native and mutant immunotoxins with patient serum. The concentration of HA22 (●), HA22-LR (○), HA22-LR-8M (△), and HA22-LR-LO10 (□) at which binding of antibodies to SS1P was inhibited by 50% (IC50) is 85, 38, 3,610, and 396,000 pM, respectively. Using the IC50 values we calculated the binding ratios as 223 for HA22-LR, 2.35 for HA22-LR-8M and < 0.0214% for HA22-LR-LO10. (B) Ratio of binding of mutant immunotoxins to binding of HA22 from 11 patients treated with BL22 (3), 1 with LMB9 (12), 7 with SS1P (7), and 6 with HA22 (4).