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Cyclic voltammograms showing direct electrocatalysis of H+ reduction/H2 oxidation for different hydrogenases (as indicated in each panel) attached to a PGE electrode. Conditions: pH 6 at 30 °C, ω = 2,500 rpm, ν = 5 mV s-1, 100% H2. Temperature: 22 ºC, except for CrHydA1 (18 ºC).