Figure 3.

The Inhibition of the Preferential Degradation of the mt− cpDNA in bp31.

(A) DIC ([a], [c], [e], [g], [i], and [k]) and fluorescent ([b], [d], [f], [h], [j], and [l]) images of SYBR Green I–stained young zygotes. N, nucleus; arrow, cp nucleoid; arrowhead, mt nucleoid; red autofluorescence indicates chlorophyll. In wild-type (WT) mt+ × wild-type mt− (left panel), the mt− cp nucleoids were digested for ∼60 min after mating. By contrast, the mt− cp nucleoids remained even after the fusion of the cell nuclei in bp31 mt+ × wild-type mt− (left panel). The preferential degradation of mt− cpDNA was not observed in bp31.

(B) The frequencies of unmated gametes (blue), zygotes with mt− cp nucleoids (red), and zygotes lacking mt− cp nucleoids (green) at 30, 60, 90, and 120 min after mating.