Figure 6.

The Dynamic Changes in Gene Expression upon Zygote Formation Are Severely Disturbed in bp31 and Are Restored in bp31 Transformed with GSP1 and INM1.

Comparison of the expression profiles of gamete- and zygote-specific genes in wild-type (WT) mt+ × wild-type mt− (green), bp31 mt+ × wild-type mt− (pink), and bp31+GSP1g+INM1gu #14 (complemented bp31) mt+ × wild-type mt− (orange). The cDNAs were prepared from the vegetative cells and gametes of mt− and mt+ cells (V−, G−, V+, and G+) and zygotes (30, 60, and 90 min after mating) and analyzed using qRT-PCR. mt+ gamete-specific genes (FUS1 [a] and SAG1 [c]), mt− gamete-specific gene (GSM1 [b]), and zygote-specific genes (EZY1 [d], EZY2 [e], ZSP1 [f], and ZYS3 [g]) were investigated. The relative levels were normalized to the mt+ wild-type vegetative cells (normalized to 1).