Figure 7.

The bp31 Mutant Could Not Be Complemented Solely by INM1 or GSP1.

(A) DIC ([a], [c], and [e]) and fluorescent ([b], [d], and [f]) images of SYBR Green I–stained young zygotes of bp31+INM1gu mt+ × wild-type (WT) mt−. The phenotype was identical to bp31, and neither the active digestion of the mt− cpDNA nor pellicle formation was observed.

(B) A DIC image of gametes of bp31 mt+ transformed with GSP1gu mixed with wild-type mt− gametes. These gametes showed agglutination but did not form zygotes.

(C) The expression levels of GSP1, FUS1, SAG1, and INM1 in wild-type, bp31, and bp31+GSP1g gametes incubated with or without 10 mM N⁶,2′-O-dibutyryladenosine 3′,5′-cyclic monophosphate and 1 mM 3-isobutyl-1-methylxanthine, as analyzed by qRT-PCR. The gametic expression of FUS1 was suppressed almost completely in the bp31+GSP1g gametes.